Improvement in the In Vivo Digestibility of Rice Protein by Alkali Extraction Is Due to Structural Changes in Prolamin/Protein Body-I Particle
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- KUBOTA Masatoshi
- Department of Applied Biological Chemistry, Graduate School of Science and Technology, Niigata University
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- SAITO Yuhi
- Graduate School of Life and Environmental Science, Kyoto Prefectural University
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- MASUMURA Takehiro
- Graduate School of Life and Environmental Science, Kyoto Prefectural University
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- KUMAGAI Takehisa
- Kameda Seika Co., Ltd.
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- WATANABE Reiko
- Department of Health and Nutrition, University of Niigata Prefecture
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- FUJIMURA Shinobu
- Department of Applied Biological Chemistry, Graduate School of Science and Technology, Niigata University
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- KADOWAKI Motoni
- Department of Applied Biological Chemistry, Graduate School of Science and Technology, Niigata University
Bibliographic Information
- Other Title
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- Improvement in the in vivo digestibility of rice protein by alkali extraction is due to structural changes in prolamin/protein body-1 particle
- Improvement in the<i>In Vivo</i>Digestibility of Rice Protein by Alkali Extraction Is Due to Structural Changes in Prolamin/Protein Body-I Particle
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Abstract
Rice prolamin, constituting type-I protein body (PB-I), is indigestible and causes deterioration of rice protein nutritional quality. In this study, the in vivo digestibility of rice protein isolates was investigated by tracing their intraluminal transit in the gastrointestinal (GI) tract of rats by western blotting and by observing the structures excreted in the feces by electron microscopy. Two types of rice protein isolates, produced by alkali extraction (AE-RP) and by starch degradation (SD-RP), were compared. The protein patterns in the isolates were similar, but their digestion in the GI-tract showed striking differences. In the AE-RP group, 13-kDa prolamin (13P) quickly disappeared in the lower GI tract and was not excreted in the feces. By contrast, in the SD-RP group, 13P accumulated massively and nearly intact PB-Is were excreted. These results indicate that the in vivo digestibility of prolamin can be improved by alkali extraction through structural changes to it.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (3), 614-619, 2010
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681456766208
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- NII Article ID
- 10027553004
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3c3gtFeksA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 10636535
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- PubMed
- 20208351
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed