Regulation of Lankamycin Biosynthesis in<i>Streptomyces rochei</i>by Two SARP Genes,<i>srrY</i>and<i>srrZ</i>
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- SUZUKI Toshihiro
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- MOCHIZUKI Susumu
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- YAMAMOTO Shouji
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- ARAKAWA Kenji
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- KINASHI Haruyasu
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
書誌事項
- タイトル別名
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- Regulation of Lankamycin Biosynthesis in Streptomyces rochei by Two SARP Genes, srrY and srrZ
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Transcription and complementation experiments were carried out to analyze the regulatory hierarchy of two Streptomyces antibiotic regulatory protein (SARP) genes, srrY and srrZ, in the γ-butyrolactone (GB)-dependent regulatory cascade in Streptomyces rochei 7434AN4. The srrY gene was transcribed in the srrZ mutant, while the srrZ gene was not in the srrY mutant. The SrrY protein was specifically bound to the promoter region of srrZ, where a possible SARP binding sequence was identified 26 bp upstream of the −10 sequence. Deletion of the repeat sequences from this region abolished its SrrY binding activity. In addition, complementation of srrZ restored lankamycin production in the srrY mutant. All of these results confirm that the SARP gene srrY directly regulates expression of the second SARP gene srrZ in a positive manner. This study gave the first confirmation of direct regulation of two SARP genes in the GB-dependent regulatory cascade in Streptomyces.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (4), 819-827, 2010
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206478787712
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- NII論文ID
- 10027554042
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10656544
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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