Purification and Characterization of Membrane-Bound 3-Dehydroshikimate Dehydratase from<i>Gluconobacter oxydans</i>IFO 3244, A New Enzyme Catalyzing Extracellular Protocatechuate Formation

• SHINAGAWA Emiko

  Department of Chemical and Biological Engineering, Ube National College of Technology

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• YAKUSHI Toshiharu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

書誌事項

タイトル別名

• Purification and Characterization of Membrane-Bound 3-Dehydroshikimate Dehydratase from Gluconobacter oxydans IFO 3244, A New Enzyme Catalyzing Extracellular Protocatechuate Formation

抄録

3-Dehydroshikimate dehydratase (DSD) is the first known enzyme catalyzing aromatization from 3-dehydroshikimate (DSA) to protocatechuate (PCA). Differently from cytosolic DSD (sDSD), a membrane-bound 3-dehydroshikimate dehydratase (mDSD) was found for the first time in the membrane fraction of Gluconobacter oxydans IFO 3244, and DSA was confirmed to be the direct precursor of PCA. In contrast to weak and instable sDSD, the abundance of mDSD in the membrane fraction suggested the metabolic significance of mDSD as the initial step in aromatization. mDSD was solubilized only by a detergent and was readily purified to high homogeneity. Its molecular weight was estimated to be 76,000. Purified mDSD showed a sole peak at 280 nm in the absorption spectrum and no critical cofactor requirements. The Km of DSA was measured at 0.5 mM, and the optimum pH was observed at pH 6–8. mDSD appeared to react only with DSA, and was inert to other compounds, such as 3-dehydroquinate, quinate, and shikimate.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 74 (5), 1084-1088, 2010

  公益社団法人 日本農芸化学会

キーワード

• acetic acid bacteria
• 3-dehydroshikimate dehydratase
• enzymatic aromatization
• oxidative fermentation
• shikimate pathway

詳細情報

• CRID: 1390001206478065792
• NII論文ID: 10027555519
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.100043
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10688989
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10688989
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.100043
• 本文言語コード: en
• データソース種別:
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