A Novel Material Incorporation Technique for Medaka (Oryzias latipes) Eggs Using Nanosecond Pulsed Electric Fields

• TOMINAGA Nobuaki

  Department of Chemical and Biological Engineering, Ariake National College of Technology

• KONO Susumu

  Department of Electrical Engineering, Ariake National College of Technology

• YAMAGUCHI Akemi

  Department of Chemical and Biological Engineering, Ariake National College of Technology

• AKIYAMA Hidenori

  Graduate School of Science and Technology, Kumamoto University

• ARIZONO Koji

  Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto

Bibliographic Information

Other Title

• A Novel Material Incorporation Technique for Medaka (<i>Oryzias latipes</i>) Eggs Using Nanosecond Pulsed Electric Fields

Abstract

The effects of nanosecond pulsed electric fields (nsPEFs) on medaka eggs were examined. Although embryogenesis was not affected by nsPEF treatment alone, significant harmful effects were observed when the eggs were treated with nsPEFs in the presence of cycloheximide in the outer solution. Nanosecond PEF treatment affected the permeability of both the egg envelope (chorion) and the cell membrane, which resulted in intracellular incorporation of cycloheximide.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 74 (6), 1279-1282, 2010

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• nanosecond pulsed electric field
• medaka
• embryogenesis
• Blumeline line generator
• electroporation

Details

• CRID: 1390282681455908736
• NII Article ID: 10027556606
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.90958
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 10734238
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10734238
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.90958
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed