Effects of Depletion of RNA-Binding Protein Tex on the Expression of Toxin Genes in Clostridium perfringens

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Author(s)

Abstract

Tex was originally identified in <I>Bordetella pertussis</I>, where it serves as a transcriptional regulator of toxin genes. However, the Tex of <I>Streptococcus pneumoniae</I> has no regulatory function in the expression of the pneumococcal major toxin pneumolysin. Here, we identified the CPE2168 gene as Tex in <I>Clostridium perfringens</I>, and examined the roles of Tex in toxin gene expression. We found that the deletion mutant for Tex does not affect growth, but the mRNA levels of three hyaluronidase genes (<I>nagH</I>, <I>nagJ</I>, and <I>nagL</I>) and an exo-sialidase (<I>nanJ</I>) were reduced to less than 50% as compared to the parent strain, <I>C. perfringens</I> strain 13. On the other hand, Tex did not affect the expression of proteases, enterotoxins, hemolysins, either of two hyaluronidase genes (<I>nagI</I> and <I>nagK</I>), an exo-sialidase (<I>nanI</I>), or adhesins. Moreover, purified Tex bound to the 5′-portion of target gene mRNAs. Based on these results, we propose that Tex positively regulates the gene expression of a set of toxin genes in <I>C. perfringens</I>.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 74(8), 1564-1571, 2010-08-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  16

Codes

  • NII Article ID (NAID)
    10027557979
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    10836416
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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