Purification and Characterization of Membrane-Associated Hydrogenase from the Deep-Sea Epsilonproteobacterium<i>Hydrogenimonas thermophila</i>

• NISHIMURA Hiroshi

  Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• KITANO Yuki

  Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• INOUE Takahiro

  Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• NOMURA Keigo

  Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• SAKO Yoshihiko

  Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

書誌事項

タイトル別名

• Purification and Characterization of Membrane-Associated Hydrogenase from the Deep-Sea Epsilonproteobacterium Hydrogenimonas thermophila

抄録

Membrane-associated hydrogenase was purified from the chemolithoautotrophic epsilonproteobacterium Hydrogenimonas thermophila at 152-fold purity. The hydrogenase was found to be localized in the periplasmic space, and was easily solubilized with 0.1% Triton X-100 treatment. Hydrogen oxidation activity was 1,365 μmol H₂/min/mg of protein at 80 °C at pH 9.0, with phenazine methosulphate as the electron acceptor. Hydrogen production activity was 900 μmol H₂/min/mg of protein at 80 °C and pH 6.0, with reduced methyl viologen as the electron donor. The hydrogenase from this organism showed higher oxygen tolerance than those from other microorganisms showing hydrogen oxidation activity. The structural genes of this hydrogenase, which contains N-terminal amino acid sequences from both small and large subunits of purified hydrogenase, were successfully elucidated. The hydrogenase from H. thermophila was found to be phylogenetically related with H₂ uptake hydrogenases from pathogenic Epsilonproteobacteria.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 74 (8), 1624-1630, 2010

  公益社団法人 日本農芸化学会

キーワード

• hydrogenase
• epsilonproteobacterium
• deep-sea hydrothermal vent
• oxygen tolerance
• H<SUB>2</SUB> production

詳細情報

• CRID: 1390001206477310592
• NII論文ID: 10027558242
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.100231
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10836493
• Web Site: http://id.ndl.go.jp/bib/10836493
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10836493
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.100231
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可