Sulfite Oxidation Catalyzed by<i>aa</i>₃-Type Cytochrome<i>c</i>Oxidase in<i>Acidithiobacillus ferrooxidans</i>

• SUGIO Tsuyoshi

  Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

• AKO Ami

  Faculty of Agriculture, Okayama University

• TAKEUCHI Fumiaki

  Environmental Management Center, Okayama University

書誌事項

タイトル別名

• Sulfite Oxidation Catalyzed by aa3-Type Cytochrome c Oxidase in Acidithiobacillus ferrooxidans

抄録

Sulfite is produced as a toxic intermediate during Acidithiobacillus ferrooxidans sulfur oxidation. A. ferrooxidans D3-2, which posseses the highest copper bioleaching activity, is more resistant to sulfite than other A. ferrooxidans strains, including ATCC 23270. When sulfite oxidase was purified homogeneously from strain D3-2, the oxidized and reduced forms of the purified sulfite oxidase absorption spectra corresponded to those of A. ferrooxidans aa₃-type cytochrome c oxidase. The confirmed molecular weights of the α-subunit (52.5 kDa), the β-subunit (25 kDa), and the γ-subunit (20 kDa) of the purified sulfite oxidase and the N-terminal amino acid sequences of the γ-subunit of sulfite oxidase (AAKKG) corresponded to those of A. ferrooxidans ATCC 23270 cytochrome c oxidase. The sulfite oxidase activities of the iron- and sulfur-grown A. ferrooxidans D3-2 were much higher than those cytochrome c oxidases purified from A. ferrooxidans strains ATCC 23270, MON-1 and AP19-3. The activities of sulfite oxidase purified from iron- and sulfur-grown strain D3-2 were completely inhibited by an antibody raised against a purified A. ferrooxidans MON-1 aa₃-type cytochrome c oxidase. This is the first report to indicate that aa₃-type cytochrome c oxidase catalyzed sulfite oxidation in A. ferrooxidans.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 74 (11), 2242-2247, 2010

  公益社団法人 日本農芸化学会

キーワード

• <I>Acidithiobacillus ferrooxidans</I>
• sulfite oxidase
• <I>aa</I><SUB>3</SUB>-type cytochrome oxidase
• bioleaching

詳細情報

• CRID: 1390001206479438080
• NII論文ID: 10027561458
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.100446
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10899760
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10899760
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.100446
• 本文言語コード: en
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