発光オワンクラゲ由来の新規マーカーGFPとトランスジェニックマウスへの応用

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  • Green Fluorescent Protein as a Novel Vital Marker in Transgenic Mouse
  • 発光オワンクラゲ由来の新規マーカーGFPとトランスジェニックマウスの応用
  • ハッコウ オワンクラゲ ユライ ノ シンキ マーカーGFP ト トランスジェニ

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The markers that have been frequently used in many researches are basically based on their enzymatic activities. Therefore, prior to the detection of the markers, we needed to add substrate to the system. In case of the observation of thin section of the organs, the addition of the substrate will not be a big obstacle in the research. In order to detect the expression of the reporter gene, newly designed substrates were developed and available in the market to allow us to stain the live cells or organs. However, the loading the substrate into cell makes it a difficult procedure to stain the live cells by these enzyme markers. A green fluorescent protein (GFP) was reported as a new type of marker especially in marking the live cells by Chalfie in 1994. The GFP emits the green fluorescent light in response to ex citation light without any pretreatment. This nature will enable us to observe the marked object in real time and in continuous manner. From this idealistic nature of GFP, many researchers are now trying to use the GFP in versatile systems as a vital marker. In the present review, we introduce the basics of GFP and our examples of using GFP in transgenic mouse lines together with a newly discovered developing usage of GFP.<br>

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