Direct Detection of Mutant DNA in a Mixed Population of Higher Copy Number Wild-Type DNA Based on Ligase Detection Reaction in Conjunction with Fluorescence Resonance Energy Transfer

DOI PDF Web Site Web Site Web Site 被引用文献3件 参考文献31件
  • HASHIMOTO Masahiko
    Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
  • YOSHIDA Kazuma
    Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
  • TSUKAGOSHI Kazuhiko
    Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

この論文をさがす

抄録

We have developed an analytical system capable of detecting point mutations in a higher copy number of wild-type DNA based on an allele-specific ligase detection reaction (LDR) in conjunction with fluorescence resonance energy transfer (FRET). Streptavidin-functionalized quantum dots (QDs) used as FRET donors effectively captured biotinylated LDR products (target DNA strands) labeled with fluorophores as a FRET acceptor, enabling the formation of a sensitive energy transfer pair and direct detection of the targets without any post-LDR separation process, which is generally required for the LDR-based mutation analysis. Our experiments indicated that the present system had an ability to detect one mutant sequence in 10 normal sequences at a signal-to-background ratio of ca. 3.9.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 26 (12), 1255-1259, 2010

    社団法人 日本分析化学会

被引用文献 (3)*注記

もっと見る

参考文献 (31)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ