Extracellular Recording from Neuronal Networks Cultured on Hydrogel-coated Microelectrode Array

• Goto Miho

  Graduate School of Frontier Sciences, The University of Tokyo

• Moriguchi Hiroyuki

  Graduate School of Frontier Sciences, The University of Tokyo

• Takayama Yuzo

  Graduate School of Information Science and Technology, The University of Tokyo

• Saito Aki

  Graduate School of Frontier Sciences, The University of Tokyo

• Kotani Kiyoshi

  Graduate School of Frontier Sciences, The University of Tokyo

• Jimbo Yasuhiko

  Graduate School of Frontier Sciences, The University of Tokyo

Bibliographic Information

Other Title

• 微小電極アレイ上のハイドロゲル膜を介した培養神経細胞の活動計測
• ビショウ デンキョク アレイ ジョウ ノ ハイドロゲル マク オ カイシタ バイヨウ シンケイ サイボウ ノ カツドウ ケイソク

Abstract

Microelectrode array (MEA) has been widely used for ensemble recording. One of the advantages of MEA recording is its capability of studying correlation between network structures and the ensemble activity-patterns. Simple neuronal networks, from which activities of individual cells can be identified, are promising for this purpose. We have developed a mask-free cell-patterning method named “micropipette drawing”. In this method, a thin hydrogel layer is formed on the surface of MEA substrates, which acts as the support for growth-guidance patterns. Here in this work, we tested whether electrical signals could be detected through this gel layer. Rat cortical neurons were cultured on substrates with guiding patterns. Electrical activities could be detected after 7 days in vitro (DIV) in both patterned and normal cell cultures, though the signal to noise ratio in the normal culture was clearly higher than that in the patterned culture. Frequency analysis demonstrated that the difference of the power spectra between these cultures was particularly significant in high frequency regions. Decreases in high-frequency components were more prominent in the signals obtained from the patterned cultures. This result suggested that the hydrogel layer acted as low-pass filters probably due to its capacitive properties. The next step is to establish a method to form hydrogel layers, which maintain growth-guidance properties and have better frequency characteristics.

Journal

• IEEJ Transactions on Electronics, Information and Systems

  IEEJ Transactions on Electronics, Information and Systems 131 (1), 29-34, 2011

  The Institute of Electrical Engineers of Japan

Keywords

• agarose-gel
• micropipette drawing
• microelectrode array
• extracellular recording
• cultured neuronal network

Details

• CRID: 1390001204607148800
• NII Article ID: 10027636567
• NII Book ID: AN10065950
• DOI: 10.1541/ieejeiss.131.29
• ISSN: 13488155
• ISSN: 03854221
• NDL BIB ID: 10929284
• Web Site: http://id.ndl.go.jp/bib/10929284
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10929284
• Web Site: http://www.jstage.jst.go.jp/article/ieejeiss/131/1/131_1_29/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed