Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

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Author(s)

    • Deng Biyang DENG Biyang
    • Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University
    • Yin Huihui YIN Huihui
    • Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University
    • Liu Yang [他] LIU Yang
    • Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University
    • NING Xi
    • Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University

Abstract

A novel method for the determination of propranolol hydrochlorid (PRO) in human urine was developed using capillary electrophoresis coupled with electrochemiluminescence detection (CE-ECL). The parameters that affected the separation and detection were optimized. Under the optimal conditions, the linear range for PRO was from 0.003 to 2 μg/mL (<i>r</i><sup>2</sup> = 0.9993), and the detection limit was 1.3 ng/mL (<i>S/N</i> = 3). The method was successfully applied to the study of the pharmacokinetics of PRO in human urine. The relative standard deviations of ECL intensity and migration time were 2.6 and 2.1%, respectively (1.0 μg/mL PRO, <i>n</i> = 6). The recovery was between 96.71 and 97.30%. The peak excretion rate in urine was observed during the 0.5 – 1 h after oral administration of a 10-mg PRO tablet and the urinary excretion ratio of PRO was 13.6% within 12 h. The method was simple, rapid, economical and sensitive, and may improve the detection of PRO as a doping agent in sports.

Journal

  • Analytical Sciences

    Analytical Sciences 27(1), 55-59, 2011-01-10

    The Japan Society for Analytical Chemistry

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Codes

  • NII Article ID (NAID)
    10027739599
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09106340
  • NDL Article ID
    10932899
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z54-F482
  • Data Source
    CJP  NDL  J-STAGE 
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