Spontaneous Calcium Changes in Micro Neuronal Networks
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- Saito Aki
- Graduate School of Frontier Sciences, University of Tokyo
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- Moriguchi Hiroyuki
- Graduate School of Frontier Sciences, University of Tokyo
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- Iwabuchi Shin
- Graduate School of Frontier Sciences, University of Tokyo
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- Goto Miho
- Graduate School of Frontier Sciences, University of Tokyo
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- Takayama Yuzo
- Graduate School of Frontier Sciences, University of Tokyo
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- Kotani Kiyoshi
- Graduate School of Frontier Sciences, University of Tokyo
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- Jimbo Yasuhiko
- Graduate School of Frontier Sciences, University of Tokyo
Bibliographic Information
- Other Title
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- 微小神経回路における自発カルシウム濃度変化
- ビショウ シンケイ カイロ ニ オケル ジハツ カルシウム ノウド ヘンカ
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Abstract
We have developed a practical experimental method to mass-produce and maintain a variation of minimal neuronal networks (“micro neuronal networks”) consisted of a single to several neurons in culture using spray-patterning technique. In this paper, we could maintain the micro-cultures for one month or more by adding conditioned medium and carried out optical recording of spontaneous activity in micro neuronal networks and considered the interactions between them. To determine the interactions between micro neuronal networks, fluorescence changes in several small networks were simultaneously measured using calcium indicator dye fluo-4 AM, and time-series analysis was carried out using surrogate arrangements. By using the spray-patterning method, a large number of cell-adhesive micro regions were formed. Neurons extended neurites along the edge of the cell-adhesive micro regions and form micro neuronal networks. In part of micro regions, some neurite was protruded from the region, and thus micro neuronal networks were connected with synapses. In these networks, a single neuron-induced network activity was observed. On the other hand, even in morphologically non-connected micro neuronal networks, synchronous oscillations between micro neuronal networks were observed. Our micro-patterning methods and results provide the possibility that synchronous activity is occurred between morphologically non-connected neuronal networks. This suggest that the humoral factor is also a important component for network-wide dynamics.
Journal
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- IEEJ Transactions on Electronics, Information and Systems
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IEEJ Transactions on Electronics, Information and Systems 131 (3), 613-618, 2011
The Institute of Electrical Engineers of Japan
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Details 詳細情報について
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- CRID
- 1390001204609446016
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- NII Article ID
- 10027804470
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- NII Book ID
- AN10065950
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- ISSN
- 13488155
- 03854221
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- NDL BIB ID
- 10986383
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed