Cationic Comb-type Copolymers Do Not Cause Collapse but Shrinkage of DNA Molecules

• Naotaka Sonda

  Institute for Materials Chemistry and Engineering, Kyushu University

• Masanori Hirano

  Institute for Materials Chemistry and Engineering, Kyushu University

• Naohiko Shimada

  Institute for Materials Chemistry and Engineering, Kyushu University

• Arihiro Kano

  Institute for Materials Chemistry and Engineering, Kyushu University

• Satoru Kidoaki

  Institute for Materials Chemistry and Engineering, Kyushu University

• Atsushi Maruyama

  Institute for Materials Chemistry and Engineering, Kyushu University

抄録

A flow-stretching assay was employed to elucidate polycation/DNA interactions at the single-molecule level. Bacteriophage λ-DNA (48.5 kbp) was attached at one end to a PEG-modified glass surface and stretched by buffer flow. The stretched DNA had an approximate length of 12 µm. Upon injection of polylysine homopolymer, the DNA folded into a globule structure within a few seconds. Injection of a polylysine graft copolymer having hydrophilic dextran side chains also induced collapse of the stretched DNA. In comparison to these polymers, a copolymer with higher graft content did not cause DNA collapse but rather caused 25% shrinkage of the extended DNA. These results were compared with those observed with unstretched DNA.

収録刊行物

• Chemistry Letters

  Chemistry Letters 40 (3), 250-251, 2011-02-05

  Oxford University Press (OUP)

キーワード

• General Chemistry

詳細情報

• CRID: 1360002219111527936
• NII論文ID: 10027815446
• NII書誌ID: AA00603318
• DOI: 10.1246/cl.2011.250
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/40/3/250/55604042/cl.2011.250.pdf
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