Edwardsiella tarda の線毛に対するヒラメ抗体の検出 Detection of Japanese Flounder Antibody against Fimbrial Major Protein of Edwardsiella tarda

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Abstract

E. tarda組換え線毛タンパク質を作製し、線毛に対するヒラメの抗体を検出する系を確立した。培地上で線毛を産生している菌体を腹腔内接種したヒラメから線毛に対する抗体が特異的に検出された。エドワジエラ症が発生したヒラメ養殖場では、死亡の増加とともに、線毛に対する抗体陽性魚の割合が増加した。養殖場のエドワジエラ症流行における線毛を保有する菌体の重要性が示唆された。

A recombinant fimbrial protein of <i>Edwardsiella tarda</i> was designed to detect the specific antibody in Japanese flounder <i>Paralichthys olivaceus</i>. The specific antibody was detected from the fish intraperitoneally injected with <i>E. tarda</i> expressing the fimbria on LB agar containing 3% NaCl, but not from those injected with <i>E. tarda</i> without the fimbria, <i>Streptococcus iniae, S. parauberis</i> or viral hemorrhagic septicemia virus. In a flounder farm suffered with edwardsiellosis, the antibody-positive fish increased in ration with the occurrence of mortality. These results suggested that <i>E. tarda</i> with the fimbria was important for epidemics of edwardsiellosis at flounder farms.

Journal

  • Fish Pathology

    Fish Pathology 46(1), 23-26, 2011-03-15

    The Japanese Society of Fish Pathology

References:  21

Codes

  • NII Article ID (NAID)
    10027836518
  • NII NACSIS-CAT ID (NCID)
    AN00063165
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    0388788X
  • NDL Article ID
    11051521
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z18-994
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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