Detection of Japanese Flounder Antibody against Fimbrial Major Protein of Edwardsiella tarda

DOI JASI Web Site 32 References
  • Sakai Takamitsu
    Tamaki Station, National Research Institute of Aquaculture, Fisheries Research Agency
  • Miyoshi Yasuyuki
    Fisheries Research Institute, Oita Prefectural Agriculture, Forestry and Fisheries Research Center
  • Matsuyama Tomomas
    National Research Institute of Aquaculture, Fisheries Research Agency
  • Nakayasu Chihaya
    Tamaki Station, National Research Institute of Aquaculture, Fisheries Research Agency
  • Kamaishi Takashi
    National Research Institute of Aquaculture, Fisheries Research Agency
  • Fukuda Yutaka
    Fisheries Research Institute, Oita Prefectural Agriculture, Forestry and Fisheries Research Center
  • Iida Takaji
    National Research Institute of Aquaculture, Fisheries Research Agency

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Other Title
  • Edwardsiella tardaの線毛に対するヒラメ抗体の検出

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Abstract

A recombinant fimbrial protein of Edwardsiella tarda was designed to detect the specific antibody in Japanese flounder Paralichthys olivaceus. The specific antibody was detected from the fish intraperitoneally injected with E. tarda expressing the fimbria on LB agar containing 3% NaCl, but not from those injected with E. tarda without the fimbria, Streptococcus iniae, S. parauberis or viral hemorrhagic septicemia virus. In a flounder farm suffered with edwardsiellosis, the antibody-positive fish increased in ration with the occurrence of mortality. These results suggested that E. tarda with the fimbria was important for epidemics of edwardsiellosis at flounder farms.

Journal

  • Fish Pathology

    Fish Pathology 46 (1), 23-26, 2011

    The Japanese Society of Fish Pathology

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