Development of a Sensitive Method for the Detection of Young Larvae of the Parasitic Pycnogonid Nymphonella tapetis in Manila Clam Ruditapes philippinarum

• Yoshinaga Tomoyoshi

  Department of Aquatic Bioscience, Graduate School of Agricultural Life Sciences, The University of Tokyo

• Kobayashi Yutaka

  Tokyo Bay Fisheries Laboratory, Chiba Prefectural Fisheries Research Center

• Toba Mitsuharu

  Tokyo Bay Fisheries Laboratory, Chiba Prefectural Fisheries Research Center

• Miyama Yoshifumi

  Tokyo Bay Fisheries Laboratory, Chiba Prefectural Fisheries Research Center

Bibliographic Information

Other Title

• アサリに寄生するウミグモ幼生の鋭敏な検出法の開発

Abstract

We developed a sensitive method for the detection of Nymphonella tapetis (sea spider) larvae in Manila clam Ruditapes philippinarum. The gills were removed from infected clams and lysed in 2N NaOH at 60°C. The lysate was centrifuged and the pellet was stained in 0.2% Uvitex 2B solution and observed under a fluorescent microscope. Using this method, we were easily able to enumerate the larvae. The majority of larvae were found in the gills, which were indicated as the primary site of infection for N. tepetis larvae.

Journal

• Fish Pathology

  Fish Pathology 46 (1), 38-41, 2011

  The Japanese Society of Fish Pathology

Keywords

• <i>Nymphonella tapetis</i>
• <i>Ruditapes philippinarum</i>
• detection
• Uvitex 2B

Details

• CRID: 1390001206461930496
• NII Article ID: 10027836588
• NII Article ID: 130000674865
• NII Book ID: AN00063165
• DOI: 10.3147/jsfp.46.38
• ISSN: 18817335
• ISSN: 0388788X
• NDL BIB ID: 11051595
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010810487
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11051595
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed