Distribution of Hydrogen Peroxide-dependent Reaction in a Gelatin Sample Irradiated by Carbon Ion Beam

DOI Web Site 8 Citations 27 References
  • MATSUMOTO Ken-ichiro
    Radiation Modifier Research Team, Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, Molecular Imaging Center, National Institute of Radiological Sciences
  • AOKI Ichio
    Magnetic Resonance Molecular Imaging Team, Biophysics Group, Molecular Imaging Center, National Institute of Radiological Sciences
  • NAKANISHI Ikuo
    Radiation Modifier Research Team, Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, Molecular Imaging Center, National Institute of Radiological Sciences
  • MATSUMOTO Atsuko
    Radiation Modifier Research Team, Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, Molecular Imaging Center, National Institute of Radiological Sciences
  • NYUI Minako
    Radiation Modifier Research Team, Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, Molecular Imaging Center, National Institute of Radiological Sciences
  • ENDO Kazutoyo
    Department of Physical Chemistry, Showa Pharmaceutical University
  • ANZAI Kazunori
    Radiation Modifier Research Team, Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, Molecular Imaging Center, National Institute of Radiological Sciences

Search this article

Abstract

We investigated the amount and distribution of hydrogen peroxide (H2O2) generated in a solid gelatin sample irradiated by heavy ion (carbon) beam. We irradiated the gelatin sample, which contained a nitroxyl radical (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl, TEMPOL), with a 290-MeV/nucleon carbon beam (~128 Gy). To verify the distribution of H2O2 generation in the irradiated sample, we employed both electron paramagnetic resonance (EPR) spectroscopic and magnetic resonance (MR) imaging methods based on H2O2-dependent paramagnetic loss of TEMPOL. We obtained a distribution profile of the H2O2-dependent reaction in the gelatin sample when we irradiated gelatin samples with carbon beams with several different linear energy transfer (LET) values. Because the profiles of oxygen consumption in the gelatin sample measured by L-band EPR oxymetry and of the H2O2-dependent reaction have almost the same shape, the profile of the H2O2-dependent reaction can be used as an estimation of the profile of the generation of H2O2. The H2O2 profile in one intact gelatin sample scanned by 7-tesla MR imaging showed a similar shape as a result of the EPR experiment. We obtained several hundreds of micromolars of H2O2 generated in a gelatin sample irradiated by carbon beam when 200 Gy was given at the surface of the sample. H2O2 distribution was almost flat, with only a slight peak just before the end of the beam.<br>

Journal

Citations (8)*help

See more

References(27)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top