Indirect ELISA and Indirect Immunofluorescent Antibody Assay for Detecting the Antibody against Murine Norovirus S7 in Mice

• KITAGAWA Yota

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• TOHYA Yukinobu

  Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• IKE Fumio

  Experimental Animal Division, RIKEN BioResource Center

• KAJITA Ayako

  Experimental Animal Division, RIKEN BioResource Center

• PARK Sang-Jin

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• ISHII Yoshiyuki

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• KYUWA Shigeru

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• YOSHIKAWA Yasuhiro

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo

抄録

To evaluate murine norovirus (MNV) infection in laboratory mice, we attempted to develop an enzyme-linked immunosorbent assay (ELISA) system and an indirect immunofluorescent antibody (IFA) assay for detecting the anti-MNV-S7 antibody in mice. MNV-S7, which was isolated in Japan, was used in both assays. The antigen for ELISA was prepared by ultracentrifugation of culture supernatants of RAW 264 cells infected with MNV-S7. Positive sera were obtained from 6-week-old, female C57BL/6JJcl mice inoculated orally with MNV-S7. IFA against infected RAW 264 cells was able to discriminate positive sera from negative sera. Indirect ELISA was performed using 96-well ELISA plates coated with formalin-treated MNV-S7 antigen. In this ELISA system, mouse sera obtained 2 weeks after infection or later showed significantly high OD values and were judged positive. An equal level of anti-MNV-S7 antibody response was observed in BALB/cAJcl, C57BL/6JJcl, DBA/2JJcl, and Jcl:ICR mice; whereas, C3H/HeJJcl mice demonstrated slightly lower antibody production 4 weeks after infection. We also used this ELISA system to evaluate 77 murine serum samples obtained from 15 conventional mouse rooms in research facilities in Japan and found that approximately half of the serum samples contained antibody to MNV-S7. We found that some serum samples were negative for antibodies to mouse hepatitis virus and Mycoplasma pulmonis but positive for antibody to MNV-S7. The results suggest that the MNV infection is more prevalent than other infections such as mouse hepatitis virus and Mycoplasma pulmonis in conventional mouse colonies in Japan, as is the case in other areas of the world.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 59 (1), 47-55, 2010

  公益社団法人 日本実験動物学会

キーワード

• enzyme-linked immunosorbent assay
• immunofluorescent antibody assay
• murine norovirus

詳細情報

• CRID: 1390001205044713728
• NII論文ID: 10027852595
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.59.47
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 10810884
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10810884
• Web Site: http://www.jstage.jst.go.jp/article/expanim/59/1/59_1_47/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010166942
• 本文言語コード: en
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