Phenotypic and expression analysis of a novel spontaneous myosin 6 null mutant mouse

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  • MOCHIZUKI Eiji
    Department of Bioindustry, Tokyo University of Agriculture
  • OKUMURA Kazuhiro
    Department of Bioindustry, Tokyo University of Agriculture
  • ISHIKAWA Masashi
    Department of Bioindustry, Tokyo University of Agriculture
  • YOSHIMOTO Sachi
    Department of Bioindustry, Tokyo University of Agriculture
  • YAMAGUCHI Junya
    Department of Bioindustry, Tokyo University of Agriculture
  • SEKI Yuta
    Department of Bioindustry, Tokyo University of Agriculture
  • WADA Kenta
    Department of Bioindustry, Tokyo University of Agriculture
  • YOKOHAMA Michinari
    Department of Bioindustry, Tokyo University of Agriculture
  • USHIKI Tatsuo
    Department of Anatomy, Graduate School of Medical and Dental Sciences, Niigata University
  • TOKANO Hisashi
    Department of Otolaryngology, Tokyo Medical and Dental University
  • ISHII Rie
    Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science
  • SHITARA Hiroshi
    Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science
  • TAYA Choji
    Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science
  • KITAMURA Ken
    Department of Otolaryngology, Tokyo Medical and Dental University
  • YONEKAWA Hiromichi
    Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science
  • KIKKAWA Yoshiaki
    Department of Bioindustry, Tokyo University of Agriculture Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science

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タイトル別名
  • Phenotypic and Expression Analysis of a Novel Spontaneous Myosin VI Null Mutant Mouse

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抄録

In humans, hearing is a major factor in quality of life. Mouse models are important tools for the discovery of genes responsible for genetic hearing loss, often enabling analysis of the processes that regulate the onset of deafness in humans. Thus far, at least 400 deafness mutants have been discovered in laboratory mouse populations and used in the study of deafness. Here we report the discovery of a new spontaneous recessive Rinshoken shaker/waltzer (rsv) mutant derived from our in-house C57BL/6J stock, which exhibits circling and/or head-tossing behaviour and complete lack of auditory brain response to any sound pressure. The hearing and balance phenotypes are associated with structural defects, in particular, disorganisation and fusion of stereocilia in the inner ear hair cells. Two sets of intersubspecific N2 mice were generated for the positional cloning of the rsv mutation. The mutant locus was mapped to a 4.8-Mb region of chromosome 9, which contains myosin VI (Myo6), a gene responsible for deafness in humans and Snell's waltzer mutation in mice. The rsv mutant showed reduced expressions of Myo6 mRNA and MYO6 protein in the inner ear. Moreover, no immunoreactivity was observed in the cochlear and vestibular hair cells in the rsv mutant mice. We sequenced the genomic region (30,154 bp) of Myo6, including all coding exons, a non-coding exon, UTRs and the Myo6 promoter; however, no mutation was discovered in these regions. We therefore speculate that loss of MYO6 expression might cause shaker/waltzer behaviour and deafness in the rsv mutant; also, loss of MYO6 expression might be the result of mutations in an unidentified regulatory region(s) of the gene.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 59 (1), 57-71, 2010

    公益社団法人 日本実験動物学会

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