Identification of a potent immunostimulatory oligodeoxynucleotide from Streptococcus thermophilus lacZ
Search this article
Abstract
Immunostimulatory sequences of oligodeoxynucleotides (ODNs), such as CpG ODNs, are potent stimulators of innate immunity. Here, we identified a strong immunostimulatory CpG ODN, which we named MsST, from the lac Z gene of Streptococcus (S.) thermophilus ATCC19258, and we evaluated its immune functions. In in vitro studies, MsST had a similar ability as the murine prototype CpG ODN 1555 to induce inflammatory cytokine production and cell proliferation. In mouse splenocytes, MsST increased the number of CD80+CD11c+and CD86+CD11c+ dendritic cells and CD4+CD25+ regulatory T cells. We also analyzed the effects of MsST on the expression of regulatory cytokines by real-time quantitative PCR. MsST was more potent at inducing interleukin-10 expression than the ODN control 1612, indicating that MsST can augment the regulatory T cell response via Toll-like receptor 9, which plays an important role in suppressing T helper type 2 responses. These results suggest that S. thermophilus, whose genes include a strong Immunostimulatory sequence-ODN, is a good candidate for a starter culture to develop new physiologically functional foods and feeds.
Article
ANIMAL SCIENCE JOURNAL. 80(5):597-604 (2009)
Journal
-
- ANIMAL SCIENCE JOURNAL
-
ANIMAL SCIENCE JOURNAL 80 (5), 597-604, 2009
WILEY-BLACKWELL PUBLISHING, INC
- Tweet
Details 詳細情報について
-
- CRID
- 1050001338920797056
-
- NII Article ID
- 10027887689
-
- NII Book ID
- AA11677214
-
- ISSN
- 13443941
-
- HANDLE
- 10091/16134
-
- Text Lang
- en
-
- Article Type
- journal article
-
- Data Source
-
- IRDB
- CiNii Articles