Bovine Oocytes in Secondary Follicles Grow in Medium Containing Bovine Plasma after Vitrification
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- TAKETSURU Hiroaki
- Graduate School of Agricultural Science, Kobe University
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- TAKAJO Asuka
- Graduate School of Agricultural Science, Kobe University
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- BAO Rong-Mei
- Graduate School of Agricultural Science, Kobe University
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- HAMAWAKI Atsushi
- Animal Bio-Technology Center Kobe, Livestock Improvement Association of Japan
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- YOSHIKAWA Motoichi
- Animal Bio-Technology Center Kobe, Livestock Improvement Association of Japan
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- MIYANO Takashi
- Graduate School of Agricultural Science, Kobe University
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Abstract
There has been no culture system that supports the growth of bovine oocytes for more than 2 weeks. In the present study, bovine secondary follicles were cultured for 4 weeks, and the effects of supplemented protein components and FSH in the culture medium on the growth of the oocytes were examined. The effect of vitrification of secondary follicles on the subsequent oocyte growth was also examined. Secondary follicles (150 to 200 μm in diameter) containing growing oocytes (approximately 60 μm in diameter) were dissected from ovaries and cultured in a medium supplemented with FSH (0, 25 or 50 ng/ml) and one of the following four kinds of protein components: bovine serum albumin (BSA), bovine plasma (BPL), fetal calf serum (FCS) and bovine follicular fluid (BFF). In BSA- and BPL-supplemented media with 0 or 25 ng/ml FSH, more than 50% of follicles showed no degenerative signs during culture, and oocytes significantly increased in size after 4 weeks (P<0.05). Higher percentages of granulosa cell-enclosed oocytes were recovered from the follicles cultured in BPL-supplemented media with 0 and 25 ng/ml FSH, and the oocytes grew to 90 μm or more in diameter. In FCS- and BFF-supplemented media, FSH increased the numbers of degenerating follicles. Next, vitrified-warmed secondary follicles were cultured in BPL-supplemented medium. One third of the follicles showed no degenerative signs, and the oocytes increased in diameter to 88.8 ± 3.1 μm after 4 weeks of culture. These results suggest that a BPL-supplemented medium supports oocyte growth in bovine secondary follicles for 4 weeks, even after vitrification and warming of the follicles.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 57 (1), 99-106, 2011
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390282681314494208
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- NII Article ID
- 10027901298
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 10991673
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed