Stimulation of calcium signal transduction involves in enhancement of production of scopadulcic acid B by methyl jasmonate in the cultured tissues of Scoparia dulcis

• Nkembo Marguerite Kasidimoko

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical Univeristy

• Kurosaki Fumiya

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical Univeristy

• Lee Jung-Bum

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical Univeristy

• Hayashi Toshimitsu

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical Univeristy

書誌事項

タイトル別名

• Stimulation of calcium signal transduction results in enhancement of production of scopadulcic acid B by methyl jasmonate in the cultured tissues of Scoparia dulcis

抄録

Signal transduction events which are involved in methyl jasmonate (MeJA)-enhanced production of the tetracyclic diterpene, scopadulcic acid B (SDB), were investigated in leaf organ cultures of Scoparia dulcis. Pretreatment of leaf organ cultures with Ca²⁺-channel blocker, verapamil, resulted in a dose-dependent inhibition of MeJA enhanced SDB production. Treatments with Ca²⁺ ionophore, A23187, stimulated SDB production in the absence of MeJA. In addition, preincubation of the leaf organ cultures with calmodulin (CaM) antagonist, trifluoperazine (TFP), diminished MeJA enhanced SDB production. MeJA-enhanced production of SDB was suppressed by addition of protein kinase inhibitors such as staurosporine and 2,6-diamino-N-([1-(1-oxotridecyl)-2-piperdinyl]methyl)hexanamide (NPC-15437). An activator of protein kinase C, 1-oleoyl-2-acetyl-sn-glycerol (OAG), stimulated this production in the absence of MeJA. These results indicate that Ca²⁺ and CaM mediates signaling events leading to enhancement of SDB production upon MeJA treatment. It was also suggested that phosphorylation of protein by protein kinases might be involved in this signal pathway.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 22 (4), 333-337, 2005

  日本植物バイオテクノロジー学会

キーワード

• calmodulin
• diterpene
• methyl jasmonate
• scopadulcic acid B
• <i>Scoparia dulcis</i>

詳細情報

• CRID: 1390282679305209344
• NII論文ID: 10028054205
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.22.333
• ISSN: 13476114
• ISSN: 13424580
• ISSN: http://id.crossref.org/issn/08454213
• ISSN: http://id.crossref.org/issn/13424580
• NDL書誌ID: 7741860
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7741860
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可