Even juvenile leaves of tobacco exhibit programmed cell death

• Horii Manabu

  School of Agriculture, Ibaraki University

• Marubashi Wataru

  School of Agriculture, Meiji University

抄録

Although the relationship between leaf senescence and programmed cell death (PCD) has been actively researched, no research had been carried out previously on juvenile and young leaves. We showed evidence of PCD (DNA laddering by agarose gel electrophoresis and DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assays) for juvenile and young leaves of Nicotiana tabacum. Additionally, confocal laser scanning microscopy of nuclei in protoplasts from juvenile leaves clarified the beginning stages of nuclear fragmentation. We concluded that even juvenile leaves of tobacco exhibit PCD and that tobacco leaves grow while undergoing PCD from the initial stages of development until senescence.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 22 (4), 339-344, 2005

  日本植物バイオテクノロジー学会

キーワード

• confocal laser scanning microscopy
• juvenile leaves
• <i>Nicotiana tabacum</i>
• programmed cell death
• TUNEL

詳細情報

• CRID: 1390282679305210624
• NII論文ID: 10028054230
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.22.339
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 7741873
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7741873
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可