Stress Tolerance and Symbiotic and Phylogenic Features of Root Nodule Bacteria Associated with Medicago Species in Different Bioclimatic Regions of Tunisia

• Djedidi Salem

  United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology Laboratory of Plant Adaptation to Abiotic Stresses (LAPSA), Biotechnology Centre of Borj Cedria

• Yokoyama Tadashi

  Institute of Agriculture, Tokyo University of Agriculture and Technology

• Ohkama-Ohtsu Naoko

  Women’s Future Development Organization, Tokyo University of Agriculture and Technology

• Risal Chandra Prasad

  United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology

• Abdelly Chedly

  Laboratory of Plant Adaptation to Abiotic Stresses (LAPSA), Biotechnology Centre of Borj Cedria

• Sekimoto Hitoshi

  Faculty of Agriculture, Utsunomiya University

Abstract

Thirty two rhizobial isolates were obtained from different bioclimatic regions of Tunisia using as trap plants, Medicago sativa, Medicago ciliaris, Medicago polymorpha and Medicago minima. To study their diversity and characterize them in relation to Mediterranean conditions, abiotic stress resistance, symbiotic properties and genetic diversity in terms of 16S rRNA and nodA sequences were assessed. Five isolates from M. sativa, three from M. ciliaris and three from M. minima could grow at 45°C. Only two isolates from M. sativa grew at 4% NaCl. The most stress tolerant isolates were obtained from arid soils. A phylogenetic analysis of 16S rRNA genes revealed 29 isolates to be closely related to Ensifer including one (Pl.3-9) that showed a 16S rRNA sequence similar to that of Ensifer meliloti and nodA sequence similar to that of Ensifer medicae. However, three isolates were categorized into Agrobacterium containing the nodA of Ensifer. Furthermore, these isolates developed nodules on original hosts. The results for the four isolates suggest horizontal gene transfer between the species.<br>

Journal

• Microbes and Environments

  Microbes and Environments 26 (1), 36-45, 2011

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• <i>Ensifer</i>
• stress tolerance
• Tunisia
• phylogeny
• symbiotic properties

Details

• CRID: 1390001204345118336
• NII Article ID: 10028098815
• NII Article ID: 130000435294
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me10138
• ISSN: 13474405
• ISSN: 13426311
• ISSN: http://id.crossref.org/issn/13474405
• NDL BIB ID: 10991305
• Web Site: http://id.ndl.go.jp/bib/10991305
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10991305
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/26/1/26_ME10138/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed