Multiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium

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Author(s)

    • PUI Chai Fung
    • Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
    • WONG Woan Chwen
    • Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
    • CHAI Lay Ching
    • Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
    • LEE Hai Yen
    • Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
    • NOORLIS Ahmad
    • Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
    • TUAN ZAINAZOR Tuan Chilek
    • Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
    • GHAZALI Farinazleen Mohamad
    • Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
    • CHEAH Yoke Kqueen
    • Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
    • RADU Son
    • Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia

Abstract

Salmonellosis outbreaks involving typhoid fever and human gastroenteritis are important diseases in tropical countries where hygienic conditions are often not maintained. A rapid and sensitive method to detect <I>Salmonella</I> spp., <I>Salmonella</I> Typhi and <I>Salmonella</I> Typhimurium is needed to improve control and surveillance of typhoid fever and <I>Salmonella</I> gastroenteritis. Our objective was the concurrent detection and differentiation of these food-borne pathogens using a multiplex PCR. We therefore designed and optimized a multiplex PCR using three specific PCR primer pairs for the simultaneous detection of these pathogens. The concentration of each of the primer pairs, magnesium chloride concentration, and primer annealing temperature were optimized before verification of the specificity of the primer pairs. The target genes produced amplicons at 429 bp, 300 bp and 620 bp which were shown to be 100% specific to each target bacterium, <I>Salmonella</I> spp., <I>Salmonella</I> Typhi and <I>Salmonella</I> Typhimurium, respectively.

Journal

  • Tropical Medicine and Health

    Tropical Medicine and Health 39(1), 9-15, 2011-03-01

    Japanese Society of Tropical Medicine

References:  23

Codes

  • NII Article ID (NAID)
    10028116651
  • NII NACSIS-CAT ID (NCID)
    AA11912846
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13488945
  • Data Source
    CJP  IR  J-STAGE 
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