ブタ原始卵胞の生存性に及ぼす KIT ligand の影響 Effect of KIT Ligand on the Viability of Porcine Primordial Follicles In Vitro

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Abstract

哺乳類の卵胞の発達開始を制御する機構については不明な点が多い。本研究では,原始卵胞の生存性,活性化および発達に及ぼすKIT ligand(KL)の影響を検討した。原始卵胞を含むブタ卵巣組織片をKL(10,100,250および500ng/ml)添加培養液中で7日間培養したところ,対象区(56±1%)に比べて,100ng/ml KL添加培養液中(39±2%)で原始卵胞の退行率は有意に低下した。より高濃度のKLでは,原始卵胞は退行した。しかし,いずれの濃度においても原始卵胞は発達を開始しなかった。次いで,卵巣組織片を10 -6 Mテストステロンあるいは10 -6 Mテストステロン+100ng/ml KL添加培養液中で培養した。テストステロン+KL添加培養液中では5±3%が初期二次卵胞へと発達したが,発達を開始した全卵胞の割合は(28±5%),テストステロン単独添加(21±5%)の場合と有意な差はなかった。以上の結果から,本実験で用いた培養条件下では,KLは卵胞の生存性を維持するが,原始卵胞の活性化を誘起しないことが示唆された。

In mammals, the mechanisms regulating the initiation of follicle development remain poorly understood. We previously reported that testosterone induced the activation of porcine primordial follicles after 7 days of culture. Herein, the effect of KIT ligand (KL) on the viability, activation and development of porcine primordial follicles was examined. Ovarian strips containing primordial follicles were cultured for 7 days in a medium supplemented with KL (10, 100, 250 or 500 ng/ml). The percentage of degenerated follicles was significantly decreased (39 ± 2%) in the 100 ng/ml KL-treated group compared to the control (56 ± 1%), whereas almost all of the primordial follicles degenerated at higher concentrations of KL. No primordial follicles developed in culture at any concentration of KL. When ovarian strips were cultured in medium supplemented with testosterone (10<sup>–6</sup> M) + KL (100 ng/ml), primordial follicles developed to early secondary follicles (5 ± 3%), however the total percentages of developing follicles in the testosterone group (21 ± 5%) and the testosterone + KL group (28 ± 5%) were not significantly different. These results suggest that KL does not promote primordial follicle activation, although it does promote follicle viability in culture.<br>

Journal

  • Journal of Mammalian Ova Research

    Journal of Mammalian Ova Research 28(1), 61-67, 2011-04-01

    JAPANESE SOCIETY OF OVA RESEARCH

References:  28

Codes

  • NII Article ID (NAID)
    10028124082
  • NII NACSIS-CAT ID (NCID)
    AN10548943
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13417738
  • NDL Article ID
    11105098
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z18-1673
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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