Effect of KIT Ligand on the Viability of Porcine Primordial Follicles In Vitro

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  • Magamage Manjula P. S.
    Laboratory of Reproductive Biology and Biotechnology, Graduate School of Agricultural Science, Kobe University
  • Moniruzzaman Mohammad
    Laboratory of Reproductive Biology and Biotechnology, Graduate School of Agricultural Science, Kobe University
  • Miyano Takashi
    Laboratory of Reproductive Biology and Biotechnology, Graduate School of Agricultural Science, Kobe University

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  • ブタ原始卵胞の生存性に及ぼすKIT ligandの影響

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Abstract

In mammals, the mechanisms regulating the initiation of follicle development remain poorly understood. We previously reported that testosterone induced the activation of porcine primordial follicles after 7 days of culture. Herein, the effect of KIT ligand (KL) on the viability, activation and development of porcine primordial follicles was examined. Ovarian strips containing primordial follicles were cultured for 7 days in a medium supplemented with KL (10, 100, 250 or 500 ng/ml). The percentage of degenerated follicles was significantly decreased (39 ± 2%) in the 100 ng/ml KL-treated group compared to the control (56 ± 1%), whereas almost all of the primordial follicles degenerated at higher concentrations of KL. No primordial follicles developed in culture at any concentration of KL. When ovarian strips were cultured in medium supplemented with testosterone (10–6 M) + KL (100 ng/ml), primordial follicles developed to early secondary follicles (5 ± 3%), however the total percentages of developing follicles in the testosterone group (21 ± 5%) and the testosterone + KL group (28 ± 5%) were not significantly different. These results suggest that KL does not promote primordial follicle activation, although it does promote follicle viability in culture.<br>

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