PKH26 is an excellent retrograde and anterograde fluorescent tracer characterized by a small injection site and strong fluorescence emission
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- Kawaguchi Koji
- Division of Anatomy and Developmental Neurobiology, Kobe University Graduate School of Medicine
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- Katsuyama Yu
- Division of Anatomy and Developmental Neurobiology, Kobe University Graduate School of Medicine
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- Kikkawa Satoshi
- Division of Anatomy and Developmental Neurobiology, Kobe University Graduate School of Medicine
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- Setsu Tomiyoshi
- Division of Anatomy and Developmental Neurobiology, Kobe University Graduate School of Medicine
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- Terashima Toshio
- Division of Anatomy and Developmental Neurobiology, Kobe University Graduate School of Medicine
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Abstract
The fluorescent dye PKH26, which binds mainly to the cell membrane, has long stability that enables the tracing of PKH26-labeled transplanted cells in host tissue. In the present study, we examined whether this fluorescent dye works as a retrograde or anterograde tracer to label neural networks within the central nervous system of adult and postnatal day 3 (P3) mice. A small injection of the dye into the medullospinal junction resulted in the retrograde labeling of corticospinal tract (CST) neurons in layer V of the sensory-motor cortex both in the adult mice and pups. Injection of the dye into the motor cortex of the P3 pups resulted in the anterograde labeling of CST fibers at a single fiber resolution level, although a similar injection of the dye into the motor cortex of the adult mice failed to stain CST fibers anterogradely. These results suggest that, while PKH26 works as a retrograde or anterograde tracer, anterograde labeling of the adult tracts can not be expected.
Journal
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- Archives of Histology and Cytology
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Archives of Histology and Cytology 73 (2), 65-72, 2010
International Society of Histology and Cytology
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Details 詳細情報について
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- CRID
- 1390282681389810560
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- NII Article ID
- 130004462749
- 10028160064
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- NII Book ID
- AA1068990X
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- ISSN
- 13491717
- 09149465
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed