フローサイトメトリーによるマイクロパーティクル計測の標準化に関する検討

• 野村 昌作

  関西医科大学　医学部　内科学第1講座

• 稲見 則仁

  すずらん病院　循環器内科

• 藤田 真也

  関西医科大学　医学部　内科学第1講座

• 小笹 亮太郎

  関西医科大学　医学部　内科学第1講座

• 東 秀二

  日本光電工業　検体機器技術センター

• 永井 豊

  日本光電工業　検体機器技術センター 臨床・検査標準化協議会　血液検査標準化検討委員会

書誌事項

タイトル別名

• Proposal of standardization for micro-particle determination by flow cytometry

抄録

Since Wolf found platelet dust or platelet vesicle which was a bleb from activated platelet in 1960s, it has become to be known well that this vesicle has coagulant activity and recently is termed as microparticles. It is well known that microparticles are generated from many kinds of activated and/or apoptotic cells and suggested that micoparticles increase in diabetes mellitus, metabolic syndrome and coronary syndrome. In the present study, we measured the number of platelet-derived microparticles (PDMP) and that of endothelium-derived microparticles (EDMP) from healthy subjects (n=29) and patient subjects (n=20) who received percutaneous coronary intervention operation by using of flow cytometer (FCM) with FITC labeled CD41 monoclonal antibody or CD144 polyclonal antiserum for the purpose of proposing standardization of PDMP and EDMP determination. PDMP gate was set to include particles of >0.5μm and <1.0μm in forward scatter (FS) and EDMP gate was set to include particles of >0.5μm and <2.0μm in FS. FITC positive events in the gate of FS-FL1 scatter were counted by using of FCM. PDMP were found even in the healthy subjects (113±78events/μl) but numerous PDMP were found in the patient subjects (490±660events/μl). EDMP were rare in the healthy subjects (1.5±0.9events/μl) but substantial number of EDMP were found in the patient subjects (10.0±6.0events/μl). Events detected by isotype control staining were very fewer than PDMP and EDMP. These data suggest that our gate setting is convincing for measuring PDMP and EDMP by using of FCM and we propose the present gate setting for standardization of PDMP and EDMP determination.

収録刊行物

• サイトメトリーリサーチ

  サイトメトリーリサーチ 21 (1), 71-84, 2011

  日本サイトメトリー学会

キーワード

• flow cytometry
• standardization
• Microparticle determination
• platelet-derived microparticles
• endothelium-derived microparticles

詳細情報

• CRID: 1390282679449134848
• NII論文ID: 10028165442
• NII論文ID: 130005696927
• NII書誌ID: AN10356261
• DOI: 10.18947/cytometryresearch.21.1_71
• ISSN: 24240664
• ISSN: 09166920
• Web Site: https://search.jamas.or.jp/link/ui/2011161644
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可