Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed poly(ethylene glycol) tethered-chain layer

• 吉本, 敬太郎

• 長崎, 幸夫

• Kubota, Masaki

• Yoshimoto, Keitaro

• Yuan, Xiaofei

• Nagasaki, Yukio

Abstract

Protein-immobilized substrates have been widely used in several applications for biosensing and bioseparation. Many kinds of sensors have been based on changes in colorimetric, fluorometric or luminometric signals derived from the immunoreactions of protein-immobilized sensor chips and particles. Protein-immobilized substrates, such as column packings and magnetic beads, have also been used as selective materials that can rapidly and simply separate target biomolecules in solution.

Journal

• Polymer journal

  Polymer journal 43 (5), 493-496, 2011-05

  Nature pub.

Keywords

• DNA
• magnetic beads
• PEG tethered-chain layer
• poly(ethylene glycol)
• PEG
• streptavidin
• thermal stability

Details

• CRID: 1050282677601327744
• NII Article ID: 10028221047
• NII Book ID: AA00777013
• ISSN: 00323896
• ISSN: 13490540
• DOI: 10.1038/pj.2011.5
• HANDLE: 2241/114778
• NDL BIB ID: 11087206
• Web Site: https://tsukuba.repo.nii.ac.jp/records/25853
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11087206
• Web Site: http://www.nature.com/articles/pj20115.pdf
• Web Site: http://www.nature.com/articles/pj20115
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN