A flavonol synthase gene GtFLS defines anther-specific flavonol accumulation in gentian

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Abstract

Flavonoids are important for male gametophyte development. Here we report on the flavonoid components and their biosynthetic regulation in gentian anthers. Among flavonoids, flavonols, including kaempferol, quercetin and isorhamnetin derivatives, accumulated abundantly in gentian anthers. However, flavones and anthocyanins, which are the main flavonoids accumulating in petals, were not detected. Northern blot analysis of nine flavonoid biosynthetic genes showed that the ‘early’ flavonoid biosynthetic genes were expressed in both anthers and petals, and that flavonol synthase (<i>FLS</i>) transcripts were restricted to anthers. In contrast, flavone synthase II (<i>FNSII</i>) and ‘late’ flavonoid biosynthetic genes were expressed specifically in gentian petals. To confirm anther-specific expression of <i>FLS</i>, the 5′-upstream region of <i>FL</i>S (GtFLSpro) was cloned by inverse PCR and fused to the <i>uid</i>A (GUS) reporter gene. Tobacco, <i>Arabidopsis</i> and gentian plants, transformed with the GtFLSproGUS construct, exhibited anther-specific GUS expression. Expression was observed in the tapetum and in pollen at late stages of anther development in transgenic plants. These results revealed that flavonol accumulation in gentian anthers was regulated by the spatial expression of <i>GtFLS</i>. Our results also suggest that anther-specific regulation of <i>FLS</i> is conserved among higher plants and the <i>GtFLS</i> promoter is useful for induction of specific gene expression in anthers.

Journal

  • Plant Biotechnology

    Plant Biotechnology 28(2), 211-221, 2011-03-25

    Japanese Society for Plant Cell and Molecular Biology

References:  56

Cited by:  1

Codes

  • NII Article ID (NAID)
    10028259225
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13424580
  • NDL Article ID
    11023557
  • NDL Source Classification
    ZR3(科学技術--生物学--植物)
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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