Biochemical and molecular characterization of anthocyanidin/flavonol 3-glucosylation pathways in Rosa×hybrida

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Abstract

Rose petals contain 3-glucosylated anthocyanidins and flavonols. We isolated three flavonoid 3-glucosyltransferase (UF3GT) homolog genes (<i>RhUF3GT1</i>, <i>RhUF3GT2</i>, and <i>RhUF3GT3</i>) from rose. <i>RhUF3GT1</i> encoded an amino acid sequence that is almost identical to the reported rose partial <i>UF3GT</i> homologs and highly homologous to strawberry and apple UF3GTs. Recombinant RhUF3GT1 expressed in yeast catalyzes 3-glucosylation of anthocyanidins but not flavonols. <i>RhUF3GT1</i> was not expressed in the petals of many cultivars even when anthocyanin biosynthesis was active, while it was expressed in the mature petals of cultivars that synthesize cyanidin 3-glucoside in the mature petals. RhUF3GT2 and RhUF3GT3, sharing 79% identity, exhibit only 42% and 41% identities to RhUF3GT1, respectively, and are distantly related to strawberry and apple UF3GTs. They were expressed in coordination with the <i>flavonol synthase</i> gene in the petal. The recombinant RhUF3GT2 expressed in yeast catalyzed 3-glucosylation of flavonol much more efficiently than that of anthocyanidins. We suggest that RhUF3GT2 catalyzes flavonol 3-glucosylation in rose petals and that it also contributes to accumulation of anthocyanidin 3-glucoside in the petals.

Journal

  • Plant Biotechnology

    Plant Biotechnology 28(2), 239-244, 2011-03-25

    Japanese Society for Plant Cell and Molecular Biology

References:  18

Cited by:  1

Codes

  • NII Article ID (NAID)
    10028259345
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13424580
  • NDL Article ID
    11023580
  • NDL Source Classification
    ZR3(科学技術--生物学--植物)
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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