Establishment of Intestinal Epithelial Cell Lines from Adult Mouse Small and Large Intestinal Crypts
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- IWAMOTO Taku
- Department of Applied Biological Chemistry, The University of Tokyo
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- YAMADA Kiyoshi
- Department of Applied Biological Chemistry, The University of Tokyo
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- SHIMIZU Makoto
- Department of Applied Biological Chemistry, The University of Tokyo
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- TOTSUKA Mamoru
- Department of Applied Biological Chemistry, The University of Tokyo
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Small and large intestinal epithelial cell (IEC) lines were established from adult murine intestinal crypts. Both established small and large IECs line (named aMoS7 and aMoC1 respectively) expressed epithelial markers. Similarly to IECs isolated from adult mouse intestines, the expression of major histocompatibility complex (MHC) class II molecules was induced by interferon-γ-treatment in both established cell lines. This expression of MHC class II molecules was higher in small intestinal aMoS7 cells than in large intestinal aMoC1 cells. Treatment with lipopolysaccharide and with ligands of Toll-like receptors 1, 2, 3, and 7 induced secretion of interleukin-6 from both adult IEC lines. These results suggest that the aMoS7 and aMoC1 cell lines can serve as useful tools in analyzing the immunological functions of IECs, especially in studying the IEC response to microbial components and its antigen presenting ability.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (5), 925-929, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681455000832
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- NII論文ID
- 10028272672
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11088682
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- KAKEN
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- 使用不可