レジンシーラーの細胞親和性について : スーパーボンド根充シーラーと AH Plus との比較  [in Japanese] Biocompatibility of resin-based sealers : Comparison of Superbond sealer with AH Plus  [in Japanese]

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Author(s)

    • 友清 淳 TOMOKIYO Atsushi
    • 九州大学大学院歯学研究院口腔機能修復学講座歯科保存学研究分野 Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
    • 郡 勝明 KOORI Katsuaki
    • 九州大学大学院歯学研究院口腔機能修復学講座歯科保存学研究分野 Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
    • 門野内 聡 MONNOUCHI Satoshi
    • 九州大学大学院歯学研究院口腔機能修復学講座歯科保存学研究分野 Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
    • 河野 清美 KONO Kiyomi
    • 九州大学大学院歯学研究院口腔機能修復学講座歯科保存学研究分野 Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
    • 山本 直秀 YAMAMOTO Naohide
    • 九州大学大学院歯学研究院口腔機能修復学講座歯科保存学研究分野 Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
    • 寺松 陽子 TERAMATSU Yoko
    • 九州大学大学院歯学研究院口腔機能修復学講座歯科保存学研究分野 Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University

Abstract

<p><b>Abstract : </b>Endodontic sealers require biocompatibility and insolubility as well as close sealing. In this study, we examined the biocompatibility of the resin-based sealers, Superbond sealer (SB) and AH Plus sealer (AP). Each sealer was set in disc molds. The specimens after setting for 24 hours were prepared as a fresh group, and the discs washed for 7 days after setting were used as a washed group. Proliferation and differentiation assays were performed using human periodontal ligament cells (HPDLC) cultured on these discs. HPDLC cultured on washed SB discs showed proliferation, while the growth of cells on fresh SB, washed AP, or fresh AP was almost completely inhibited. SEM observation revealed tight attachment of HPDLC on the surface of washed SB, but no cells on washed AP. The gene expression of osteocalcin in HPDLC cultured on washed SB in osteogenic differentiation medium (DM) was upregulated. These results suggested that both SB and AP inhibited the cell growth initially after setting, but 7 days of washing of SB allowed HPDLC to proliferate on it, while AP still continued to elute cytotoxic components even after washing. Furthermore, washed SB would enable HPDLC on it to differentiate into cementoblast/osteoblast-like cells when cultured in DM.</p>

Journal

  • The Journal of Japan Endodontic Association

    The Journal of Japan Endodontic Association 32(2), 97-101, 2011-05-31

    Japan Endodontic Association

References:  14

Cited by:  3

Codes

  • NII Article ID (NAID)
    10029089682
  • NII NACSIS-CAT ID (NCID)
    AA11837117
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    13478672
  • Data Source
    CJP  CJPref  J-STAGE 
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