Plant Growth-Promoting Fungus, Trichoderma koningi Suppresses Isoflavonoid Phytoalexin Vestitol Production for Colonization on/in the Roots of Lotus japonicus

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Abstract

The relationship between the colonization of <i>Lotus japonicus</i> by plant growth-promoting fungi (PGPF) and biosynthesis of the isoflavonoid phytoalexin vestitol, a major defensive response of leguminous plants, was analyzed. When PGPF including <i>Trichoderma koningi</i>, <i>Fusarium equiseti</i>, and <i>Penicillium simplicissimum</i> were inoculated onto <i>L. japonicus</i> roots, only <i>T. koningi</i> colonized the roots long-term and increased plant dry weight (126%). Microscopic observations of transverse sections of roots colonized by <i>T. koningi</i> demonstrated intercellular hyphal growth and the formation of yeast-like cells. The induction of plant defenses by fungal infections was examined by Northern analysis of genes involved in vestitol biosynthesis and HPLC of vestitol production in <i>L. japonicus</i>. Inoculation with symbiotic <i>Mesorhizobium loti</i> did not induce any accumulation of the transcripts. <i>T. koningi</i> immediately suppressed transcript levels to those induced by <i>M. loti</i>. The vestitol transuded from roots by <i>T. koningi</i> was detected at a level equivalent to that transuded by <i>M. loti</i>. Other PGPF and <i>Calonectoria ilicola</i> pathogenic to soybean but not to <i>L. japonicus</i>, stimulated continuous expression of genes and exudation of vestitol. These PGPF resembled mycorrhizal fungi in the establishment of symbiotic associations rather than fungal parasites.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 26(2), 128-134, 2011-06-01

    Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology

References:  53

Cited by:  3

Codes

  • NII Article ID (NAID)
    10029122750
  • NII NACSIS-CAT ID (NCID)
    AA11173196
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13426311
  • NDL Article ID
    11095249
  • NDL Call No.
    Z54-J644
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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