Chronological Changes in Astrocytes Induced by Chronic Electrical Sensorimotor Cortex Stimulation in Rats

• MORISHITA Takashi

  Division of Neurosurgery, Department of Neurological Surgery, Nihon University School of Medicine Division of Applied System Neuroscience, Department of Advanced Medical Science, Nihon University School of Medicine

• YAMASHITA Akiko

  Division of Applied System Neuroscience, Department of Advanced Medical Science, Nihon University School of Medicine

• KATAYAMA Yoichi

  Division of Neurosurgery, Department of Neurological Surgery, Nihon University School of Medicine

• OSHIMA Hideki

  Division of Neurosurgery, Department of Neurological Surgery, Nihon University School of Medicine Division of Morphological Anatomy, Department of Functional Morphology, Nihon University School of Medicine

• NISHIZAKI Yuji

  Department of Cardiology, Juntendo University School of Medicine

• SHIJO Katsunori

  Division of Neurosurgery, Department of Neurological Surgery, Nihon University School of Medicine Division of Applied System Neuroscience, Department of Advanced Medical Science, Nihon University School of Medicine

• FUKAYA Chikashi

  Division of Neurosurgery, Department of Neurological Surgery, Nihon University School of Medicine Division of Applied System Neuroscience, Department of Advanced Medical Science, Nihon University School of Medicine

• YAMAMOTO Takamitsu

  Division of Neurosurgery, Department of Neurological Surgery, Nihon University School of Medicine Division of Applied System Neuroscience, Department of Advanced Medical Science, Nihon University School of Medicine

抄録

Motor cortex stimulation (MCS) is a treatment option for various disorders such as medically refractory pain, poststroke hemiplegia, and movement disorders. However, the exact mechanisms underlying its effects remain unknown. In this study, the effects of long-term chronic MCS were investigated by observing changes in astrocytes. A quadripolar stimulation electrode was implanted on the dura over the sensorimotor cortex of adult rats, and the cortex was continuously stimulated for 3 hours, 1 week, 4 weeks, and 8 weeks. Immunohistochemical staining of microglia (ionized calcium-binding adaptor molecule 1 [Iba1] staining) and astrocytes (glial fibrillary acidic protein [GFAP] staining), and neuronal degeneration histochemistry (Fluoro-Jade B staining) were carried out to investigate the morphological changes following long-term chronic MCS. Iba1 staining and Fluoro-Jade B staining showed no evidence of Iba1-positive microglial changes or neurodegeneration. Following continuous MCS, GFAP-positive astrocytes were enlarged and their number increased in the cortex and the thalamus of the stimulated hemisphere. These findings indicate that chronic electrical stimulation can continuously activate astrocytes and result in morphological and quantitative changes. These changes may be involved in the mechanisms underlying the neuroplasticity effect induced by MCS.<br>

収録刊行物

• Neurologia medico-chirurgica

  Neurologia medico-chirurgica 51 (7), 496-502, 2011

  一般社団法人 日本脳神経外科学会

キーワード

• motor cortex stimulation
• neural plasticity
• trophic function
• pain
• cingulate gyrus

詳細情報

• CRID: 1390001205056998272
• NII論文ID: 10029138467
• NII書誌ID: AN00358613
• DOI: 10.2176/nmc.51.496
• ISSN: 13498029
• ISSN: 04708105
• PubMed: 21785243
• Web Site: http://www.jstage.jst.go.jp/article/nmc/51/7/51_7_496/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012074316
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可