Influence of fiber structures of a substrate on cell movement and spreading in culture

DOI 2 Citations 30 References
  • YAGUCHI Toshiyuki
    Tokyo Denki University, Ishizaka Hatoyama-machi, Hikigun Saitama
  • NONAKA Kazuhiro
    Graduate school of Tokyo Denki University, Ishizaka Hatoyama-machi, Hikigun Saitama
  • FUNAKUBO Akio
    Tokyo Denki University, Ishizaka Hatoyama-machi, Hikigun Saitama
  • FUKUI Yasuhiro
    Tokyo Denki University, Ishizaka Hatoyama-machi, Hikigun Saitama

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  • 繊維性基材の構造が細胞の移動距離に与える影響

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Abstract

Here we evaluate the influence of a substrate structures on cell migration in culture. Two types of a substrate were compared: a fibrous and a Skin-Substrate prepared using a spray method. Both types of substrates were made of polyurethane. As a control, cell behavior was also evaluated in a polystyrene dish. The mouse fibroblast cell line NIH-3T3 was used for the experiment. All three types of dish were observed by microscopy at 48-hour intervals over a period of 144hours. The extent of cell migrating was defined as the radius of the area to which the cells attached. These distances were measured from micrographs and the data shown as means±SD. We found that migration distance in the culture with the Fibrous-Substrate was 3571±334.μm compared to 1848±239 μm for the Skin-Substrate and 1490±71μm in the control. We conclude that the micro-sized structure of the Fibrous-Substrate encouraged both rapid and extensive cell migration.

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