書誌事項
- タイトル別名
-
- Analysis of Cell Population Behavior on a Culture Surface<br>Using a Particle Image Velocimetry
この論文をさがす
抄録
The construction of a system for analysis of cell population behavior during real-time monitoring is anticipated to evaluate normal cellular function noninvasively and quantitatively on scaffolds. This report presents the construction of a cell visualization system in real-time and reports measurement of cellular singular nature on the scaffolds. Two types of segmented polyurethane (SPU) scaffolds, including nanofiber (0.76±0.12 μm) and microfiber (5.79±0.46 μm) scaffolds with fiber orientation were fabricated using an electrospinning method. Mouse fibroblasts (NIH3T3) were seeded to SPU fibrous scaffolds in a culture well. The relations of the scaffold structure to the direction and migration speed of the cell population were investigated to analyze cell population behavior over the period of a week using particle image velocimetry (PIV) in real-time. The PIV analysis results demonstrated the ability to perform vector analysis at the same time for plural numbers of cells. The migration of individual cells in the population was analyzed using fibers designed with a diameter of 0.76±0.12 to 5.79±0.46 μm. Individual cells in the cell population migrated approximately 30 μm/h on the fibrous scaffolds. In conclusion, the system developed using PIV was effective for the analysis of cell population behavior.
収録刊行物
-
- ライフサポート
-
ライフサポート 22 (3), 125-131, 2010
ライフサポート学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679344800896
-
- NII論文ID
- 10029238441
- 10029066925
-
- NII書誌ID
- AA11947931
-
- ISSN
- 18845827
- 13419455
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可