Effects of Eu and Sm on Methylobacterium sp.-Growth Behaviors and Expression of Methanol Dehydrogenase-

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  • Ｍｅｔｈｙｌｏｂａｃｔｅｒｉｕｍ　ｓｐ．に及ぼすＥｕとＳｍの影響―増殖特性とメタノール脱水素酵素の発現―
  • Methylobacterium sp ニ オヨボス Eu ト Sm ノ エイキョウ ゾウショク トクセイ ト メタノール ダツスイソ コウソ ノ ハツゲン
  • -Growth Behaviors and Expression of Methanol Dehydrogenase-
  • －増殖特性とメタノール脱水素酵素の発現－

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Abstract

Eu and Sm have been widely used in high technology products. In this study the authors isolated a soil bacterium, identified as Methylobacterium sp. MAFF211642, which exhibited colonies on 1/100 nutrient agar, supplemented by 30µM Eu and Sm; the soil bacterium was found to exhibit larger colonies than those in the absence of these elements. However, when 0.5% methanol was added to the nutrient agar, only Sm was found to stimulate the growth. Other rare earth and metal elements did not affect or inhibit, regardless of the presence of methanol. Addition of both Sm and methanol to the nutrient broth increased the growth of this strain 10-fold in colony forming unit larger than when both were absent. When both methanol and Sm were added to the nutrient broth, specific activity of methanol dehydrogenase in a crude extract of the bacterium increased approximately 5.4-fold.

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