Selenite Reduction by the Thioredoxin System: Kinetics and Identification of Protein-Bound Selenide

• TAMURA Takashi

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University

• SATO Kumi

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University

• KOMORI Kentaro

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University

• IMAI Takeshi

  Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University

• KUWAHARA Mitsuhiko

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University

• OKUGOCHI Takahiro

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University

• MIHARA Hisaaki

  Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University

• ESAKI Nobuyoshi

  Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University

• INAGAKI Kenji

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University

抄録

Selenite (SeO₃²⁻) assimilation into a bacterial selenoprotein depends on thioredoxin (trx) reductase in Esherichia coli, but the molecular mechanism has not been elucidated. The mineral-oil overlay method made it possible to carry out anaerobic enzyme assay, which demonstrated an initial lag-phase followed by time-dependent steady NADPH consumption with a positive cooperativity toward selenite and trx. SDS-PAGE/autoradiography using ⁷⁵Se-labeled selenite as substrate revealed the formation of trx-bound selenium in the reaction mixture. The protein-bound selenium has metabolic significance in being stabilized in the divalent state, and it also produced the selenopersulfide (-S-SeH) form by the catalysis of E. coli trx reductase (TrxB).

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (6), 1184-1187, 2011

  公益社団法人 日本農芸化学会

キーワード

• thioredoxin reductase
• selenite
• <I>Escherichia coli</I>
• anaerobic enzyme assay
• selenopersulfide

詳細情報

• CRID: 1390001206478339072
• NII論文ID: 10029328515
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.100847
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11132032
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11132032
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.100847
• 本文言語コード: en
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