A simple, large-scale overexpression method of deriving carbon monoxide dehydrogenase 2 from thermophilic bacterium Carboxydothermus hydrogenoformans
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- INOUE Takahiro
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- YOSHIDA Takashi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- WADA Kei
- Department of Biological Sciences, Graduate School of Science, Osaka University
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- DAIFUKU Takashi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- FUKUYAMA Keiichi
- Department of Biological Sciences, Graduate School of Science, Osaka University
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- SAKO Yoshihiko
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
書誌事項
- タイトル別名
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- A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans
- A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium<i>Carboxydothermus hydrogenoformans</i>
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We established an Na2S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO2 reduction activity, of 16.9 U/mg.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (7), 1392-1394, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206476986112
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- NII論文ID
- 10029329599
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11175267
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- PubMed
- 21737917
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可