発色酵素基質培地による Nocardia seriolae のα-グルコシダーゼ活性の検出と薬剤感受性の推定 The Use of Chromogenic Media for α-Glucosidase Determination and Presumptive Drug Susceptibility Profiles in the Fish Pathogen Nocardia seriolae

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Abstract

発色酵素基質培地でN. seriolaeのα-グルコシダーゼ(α-glu)活性の検出を試みたところ、アピザイムで行った結果と一致した。2001~07年に分離されたカンパチ及びブリ由来株の内、α-glu活性陽性の27株はすべてエリスロマイシン(Em)感受性で、内18株がオキシテトラサイクリン(OTC)耐性であった。陰性の89株はすべてOTC感受性で、内81株がEm耐性であった。α-glu活性と薬剤感受性プロフィールとの関連が認められた。

The utility of chromogenic media for determination of α-glucosidase (α-glu) activity in 116 <i>Nocardia seriolae</i> isolates was investigated. In all isolates, the results obtained using chromogenic media were identical to those of the API ZYM test. All the α-glu-positive isolates (n = 27) were erythromycin (Em)-sensitive, and more than half of them (n = 18) were oxytetracycline (OTC)-resistant. On the other hand, all the α-glu-negative isolates (n = 89) were OTC-sensitive, and most of them (n = 81) were Em-resistant. These results supported the relationship between the α-glu activity and drug susceptibility profiles in <i>N. seriolae</i>. Therefore, chromogenic media can be used as a simple and reproducible one-step test to determine the α-glu activity and presumptive drug susceptibility profiles in <i>N. seriolae</i>.

Journal

  • Fish Pathology

    Fish Pathology 46(2), 62-64, 2011-06-15

    The Japanese Society of Fish Pathology

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Codes

  • NII Article ID (NAID)
    10029368699
  • NII NACSIS-CAT ID (NCID)
    AN00063165
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0388788X
  • NDL Article ID
    11170298
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z18-994
  • Data Source
    CJP  CJPref  NDL  IR  J-STAGE 
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