金属タンパク質のX線結晶構造解析 : ヘム分解酵素の反応中間体  [in Japanese] X-ray Crystallography of A Metalloprotein : A Reaction Intermediate of Heme Oxygenase  [in Japanese]

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Author(s)

    • 海野 昌喜 UNNO Masaki
    • 茨城大学フロンティア応用原子科学研究センター Frontier Research Center for Applied Atomic Sciences, Ibaraki University

Abstract

X-ray crystallographic analysis of a metalloprotein requires knowing the electronic state of the metal center, if one wants to elucidate the exact function and/or reaction mechanism. As an example, we show our recent structural analysis of the heme oxygenase reaction intermediate which is involved in the third step of the heme degradation reaction. The reaction intermediate was crystallized under anaerobic condition, and the obtained crystals were frozen into liquid nitrogen. The absorption spectra of the single crystal before and after X-ray irradiation were compared with that of the frozen solution in 100 K cold nitrogen stream. The determined structure offers the first solid evidence for the presence of a water cluster in the distal pocket of this catalytically critical intermediate. This structure combined with the QM/MM calculation supports our proposal that the biliverdin is produced via Fe-OOH verdoheme intermediate.

Journal

  • Nihon Kessho Gakkaishi

    Nihon Kessho Gakkaishi 53(3), 213-218, 2011-06-30

    The Crystallographic Society of Japan

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Codes

  • NII Article ID (NAID)
    10029371570
  • NII NACSIS-CAT ID (NCID)
    AN00188364
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    03694585
  • NDL Article ID
    11173455
  • NDL Source Classification
    ZM46(科学技術--地球科学--岩石・鉱物・鉱床)
  • NDL Call No.
    Z15-138
  • Data Source
    CJP  NDL  J-STAGE 
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