豚の全身性 Actinobacillus suis 感染症  [in Japanese] Systemic Actinobacillus suis Infection in a Piglet  [in Japanese]

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Abstract

重度の削痩が認められた30日齢の離乳豚を剖検したところ,線維素性胸膜肺炎がみられた.病理組織学的に,肺胸膜,心外膜および腎臓髄質に多数の壊死巣が認められ,その周囲を核が伸長した好中球が層状に取り巻く像が確認された.これらの病変部にはグラム陰性菌塊が認められた.同様の病変は肝臓,脾臓,腸間膜および大脳にも認められた.免疫組織化学的に,このグラム陰性桿菌は抗<i>Actinobacillus suis</i>血清に対して陽性反応を示した.病変部から病原菌の分離培養ができなかったため,ホルマリン固定パラフィン包埋組織切片からDNAを分離した.16S rRNA遺伝子の一部を増幅・塩基配列解析した結果,分離したDNAは<i>A. suis</i>と高い相同性がみられた(100%の相同性,650bp比較).以上の成績から,<i>A. suis</i>がこの子豚に壊死性胸膜肺炎,心外膜炎および腎炎を引き起こしたと考えられた.

A 30-day-old piglet showed severe emaciation. Macroscopically, fibrinous pleuropneumonia was detected. Histopathologically, necrotic lesions were found in the pleura, epicardium, and renal medulla, and were surrounded by numerous necrotic neutrophils. Gram-negative bacterial colonies were seen in the lesions. Similar lesions were also observed in the liver, spleen, mesenterium, and brain. Immunohistochemically, the bacteria were positively reacted with rabbit anti <i>Actinobacillus suis </i>serum. 16S rRNA gene segment from genomic DNA isolated from formalin-fixed paraffin-embedded tissue sections was amplified, and the base sequence analysis results indicated high homology with <i>A. suis </i>(100% similarity, based on a comparison of 650 bp). The present results indicated that <i>A. suis </i>caused necrotic pleuropneumonia, epicarditis and nephritis in the piglet.

Journal

  • Journal of the Japan Veterinary Medical Association

    Journal of the Japan Veterinary Medical Association 64(5), 381-384, 2011-05-20

    Japan Veterinary Medical Association

References:  13

Codes

  • NII Article ID (NAID)
    10029384030
  • NII NACSIS-CAT ID (NCID)
    AN00191857
  • Text Lang
    JPN
  • Article Type
    SHO
  • ISSN
    04466454
  • NDL Article ID
    11115744
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-352
  • Data Source
    CJP  NDL  IR  J-STAGE 
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