Osteoanagenesis after transplantation of bone marrow-derived mesenchymal stem cells using polyvinylidene chloride film as a scaffold

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Author(s)

    • HAMAJIMA Soichiro
    • Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University
    • HAYASHI Tatsuhide
    • Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University
    • SATO Yamato
    • Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University
    • SASAKI Keisuke
    • Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University
    • KAWAI Tatsushi
    • Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University

Abstract

The aim of this study was to develop a new cell transplantation technique for osteoanagenesis at bone defect sites. Polyvinylidene chloride (PVDC) film was evaluated because of its good biocompatibility and flexibility. We used this film as both a cell scaffold and a barrier membrane. Initially, the cell compatibility of the PVDC film for fibroblast-like cells and osteoblast-like cells was confirmed. Subsequently, bone marrow cells were obtained from rats and cultured on PVDC films in two kinds of medium. The PVDC films with bone marrow-derived mesenchymal stem cells (MSCs) were then applied to critical-sized bone defects in the calvarial bone of rats. After the transplantation, the surgical sites were dissected out and evaluated by soft X-ray radiography, micro-CT analysis and histological examinations. The bone marrow-derived MSC-transplanted rats showed greater bone regeneration than the control rats. Therefore, PVDC film is considered to be useful as a scaffold for bone regeneration.

Journal

  • Dental Materials Journal

    Dental Materials Journal 30(5), 707-716, 2011-09-01

    The Japanese Society for Dental Materials and Devices

References:  34

Codes

  • NII Article ID (NAID)
    10029480816
  • NII NACSIS-CAT ID (NCID)
    AA10443149
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    02874547
  • Data Source
    CJP  J-STAGE 
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