Porcine Amelogenin : Alternative Splicing, Proteolytic Processing, Protein-Protein Interactions, and Possible Functions

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Author(s)

    • YAMAKOSHI Yasuo
    • Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan Dental Research Lab
    • KARAKIDA Takeo
    • Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University
    • YAMAMOTO Ryuji
    • Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University
    • NAGANO Takatoshi
    • Department of Periodontology, School of Dental Medicine, Tsurumi University
    • YAMAKOSHI Yasuo
    • Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University
    • HAYAKAWA Tohru
    • Department of Dental Engineering, School of Dental Medicine, Tsurumi University
    • OIDA Shinichiro
    • Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University
    • GOMI Kazuhiro
    • Department of Periodontology, School of Dental Medicine, Tsurumi University

Abstract

We examined the adaptability of zirconia as a fixture for implants. A mouse myoblast cell line (C2C12) was seeded on Ce-TZP and titanium disks, and on poly-L-lysine-coated glass slides. Proliferation potency was determined by cell counting and mineral induction by BMP2 was studied. The osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) and alizarin red (ARS) staining. ALP activity and calcium concentrations were colorimetrically measured. The number of cells on all materials was approximately equal. ALP and ARS staining showed densely-stained images, demonstrating the induction of C2C12 cells to express the osteoblastic phenotype. RT-PCR showed that mRNA expressions of <i>type I collagen</i>, <i>osteocalcin</i>, <i>osterix</i> and ALP were up-regulated. With regard to ALP activity and calcium concentration of C2C12 cells, no significant differences were observed between Ce-TZP and titanium disks. We conclude that Ce-TZP has the biological activity comparable to titanium and has the utility as fixture of dental implants.

Journal

  • Dental Materials Journal

    Dental Materials Journal 53(3), 275-283, 2011-08-20

    The Japanese Society for Dental Materials and Devices

References:  35

Codes

  • NII Article ID (NAID)
    10029495211
  • NII NACSIS-CAT ID (NCID)
    AA11896386
  • Text Lang
    ENG
  • Article Type
    REV
  • ISSN
    13490079
  • Data Source
    CJP  J-STAGE 
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