Porcine Amelogenin: Alternative Splicing, Proteolytic Processing, Protein-Protein Interactions, and Possible Functions

• SAITO Mari

  Department of Periodontology, School of Dental Medicine, Tsurumi University

• KARAKIDA Takeo

  Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University

• YAMAMOTO Ryuji

  Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University

• NAGANO Takatoshi

  Department of Periodontology, School of Dental Medicine, Tsurumi University

• YAMAKOSHI Yasuo

  Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University

• HAYAKAWA Tohru

  Department of Dental Engineering, School of Dental Medicine, Tsurumi University

• OIDA Shinichiro

  Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University

• GOMI Kazuhiro

  Department of Periodontology, School of Dental Medicine, Tsurumi University

書誌事項

タイトル別名

• Differentiation potential of osteoblast from cultured C2C12 cells on zirconia disk

抄録

We examined the adaptability of zirconia as a fixture for implants. A mouse myoblast cell line (C2C12) was seeded on Ce-TZP and titanium disks, and on poly-L-lysine-coated glass slides. Proliferation potency was determined by cell counting and mineral induction by BMP2 was studied. The osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) and alizarin red (ARS) staining. ALP activity and calcium concentrations were colorimetrically measured. The number of cells on all materials was approximately equal. ALP and ARS staining showed densely-stained images, demonstrating the induction of C2C12 cells to express the osteoblastic phenotype. RT-PCR showed that mRNA expressions of type I collagen, osteocalcin, osterix and ALP were up-regulated. With regard to ALP activity and calcium concentration of C2C12 cells, no significant differences were observed between Ce-TZP and titanium disks. We conclude that Ce-TZP has the biological activity comparable to titanium and has the utility as fixture of dental implants.

収録刊行物

• Dental Materials Journal

  Dental Materials Journal 33 (2), 275-283, 2014

  一般社団法人　日本歯科理工学会

キーワード

• Zirconia
• Titanium
• Dental implant
• C2C12 cells
• Osteoblast

詳細情報

• CRID: 1390282679693513984
• NII論文ID: 10029495211
• NII論文ID: 130004475747
• NII書誌ID: AA11896386
• DOI: 10.4012/dmj.2013-321
• DOI: 10.2330/joralbiosci.53.275
• COI: 1:STN:280:DC%2BC2crovVeluw%3D%3D
• ISSN: 18811361
• ISSN: 18803865
• ISSN: 02874547
• ISSN: 13490079
• PubMed: 24682023
• Web Site: https://www.jstage.jst.go.jp/article/dmj/33/2/33_2013-321/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015175762
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可