The Preparation of 28Mg and Analysis of Mg Uptake in Rice Plant

• TANOI Keitaro

  Biotechnology Research Center, The University of Tokyo Graduate School of Agricultural and Life Sciences, The University of Tokyo

• SAITO Takayuki

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• IWATA Naoko

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• OHMAE Yoshimi

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• HIROSE Atsushi

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• KOBAYASHI Natsuko I.

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• IWATA Ren

  Cyclotron and Radioisotope Center, Tohoku University

• NAKANISHI Tomoko M.

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

Bibliographic Information

Other Title

• ｀２８´Ｍｇの製造とイネにおけるＭｇ吸収解析への利用
• 28Mgの製造とイネにおけるMg吸収解析への利用
• 28Mg ノ セイゾウ ト イネ ニ オケル Mg キュウシュウ カイセキ エ ノ リヨウ

Abstract

We present the preparation of ²⁸Mg to study Mg uptake manner by rice root. Since there is no convenient Mg tracer, we prepared ²⁸Mg from a pure Al target irradiated by an α-particle beam. As a result, approximately 1MBq of carrier-free and salt-free ²⁸Mg was purified. Mg uptake velocity of rice was analyzed using seedlings at the third-, fourth- and seventh-leaf stages. Prepared ²⁸Mg was applied to the rice root for a short term (15 to 30min) with low (0.1mM) or high (5.0mM) concentration of Mg in a solution. After sample was washed with cold water, ²⁸Mg distribution was detected by an imaging plate. From the analysis, the Mg uptake rate of the root in the 5.0mM Mg was approximately 6 to 7-fold higher than that in the 0.1mM. To confirm the result more in detail, the uptake analysis was also performed with various concentrations of Mg. The results indicate that Mg uptake systems concentrate Mg when the Mg concentration in the external solution is low, which suggests the high contribution of active Mg transport system in root.

Journal

• RADIOISOTOPES

  RADIOISOTOPES 60 (8), 299-304, 2011

  Japan Radioisotope Association

Keywords

• magnesium-28
• purification
• Mg uptake
• rice
• plant

Details

• CRID: 1390282679133458816
• NII Article ID: 10029508566
• NII Book ID: AN00351589
• DOI: 10.3769/radioisotopes.60.299
• COI: 1:CAS:528:DC%2BC3MXhtlSrt7bN
• ISSN: 18844111
• ISSN: 00338303
• NDL BIB ID: 11212633
• Web Site: http://id.ndl.go.jp/bib/11212633
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11212633
• Web Site: http://www.jstage.jst.go.jp/article/radioisotopes/60/8/60_8_299/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed