MESとBN.MES-Cyba[mes]コンジェニック系ラットにおける好酸球増多症に関する系統差を規定する遺伝子は第9,5,および1番染色体上に存在する Genes for Difference in Eosinophilic Phenotype between MES and BN.MES-Cyba^<mes> Rats Are on Chromosomes 9, 5, and 1

Access this Article

Author(s)

    • 西尾 綾子 NISHIO Ayako
    • Division of Laboratory Animal Research, Research Center for Human and Environmental Sciences, Shinshu University
    • MATSUMOTO Kiyoshi
    • Division of Laboratory Animal Research, Research Center for Human and Environmental Sciences, Shinshu University
    • MORI Masayuki
    • Department of Aging Biology, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine

Abstract

The Matsumoto Eosinophilia Shinshu (MES) rat strain develops hereditary blood eosinophilia due to the mutant <i>Cyba<sup>mes</sup></i> gene. In contrast, BN.MES-<i>Cyba<sup>mes</sup></i> congenic rats, in which the mutant <i>Cyba<sup>mes</sup></i> gene introduced into the background of the BN strain, have a normal blood eosinophil level despite showing robust proliferation of eosinophils in the bone marrow. However, the congenic rats manifest focal necrosis with eosinophilic infiltration in the liver, a phenotype rarely observed in the original MES rat strain. To elucidate the genetic basis for the strain differences, (MES × BN.MES-<i>Cyba<sup>mes</sup></i>)F<sub>2</sub> rats were bred, and genetic analyses of phenotypes for eosinophilia were performed. Blood and bone marrow eosinophil levels in the F<sub>2</sub> rats showed broad distributions, suggesting that the traits were under the influence of multiple genes. Genetic association studies revealed that BN-derived marker loci on chromosomes 9 and 5 were responsible for the increase in eosinophil level in the bone marrow, decrease in blood eosinophil level, and the induction of focal necrosis with eosinophilic infiltration in the liver. The BN-derived allele of the marker gene on chromosome 1 was responsible for the decrease of both bone marrow and blood eosinophil levels. These data suggest the existence of genes characterizing/distinguishing the eosinophilic phenotypes of MES and BN.MES-<i>Cyba<sup>mes</sup></i> on these chromosomes, and form the basis for positional cloning studies of the genes. These studies will advance the understanding of the mechanisms involved in eosinophil mobilization from the bone marrow and recruitment to the organs.<br>

Journal

  • Experimental Animals

    Experimental Animals 60(2), 151-160, 2011-04-01

    Japanese Association for Laboratory Animal Science

References:  39

Codes

  • NII Article ID (NAID)
    10029550320
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13411357
  • NDL Article ID
    11036657
  • NDL Source Classification
    ZS7(科学技術--医学)
  • NDL Call No.
    Z54-H752
  • Data Source
    CJP  NDL  IR  J-STAGE 
Page Top