Effects of Estrogen on PMCA 2 and 4 in Human Fibroblast-like Synovial Cells and Mouse Macrophage-like Cells

• EL-BEIALY Waleed

  Department of Oral and Maxillofacial Surgery, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan Department of Oral Pathology and Biology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan Department of Oral and Maxillofacial Surgery, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt

• GALAL Nadia

  Department of Oral and Maxillofacial Surgery, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan Department of Oral and Maxillofacial Surgery, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt Department of Molecular Cell Pharmacology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

• DEYAMA Yoshiaki

  Department of Molecular Cell Pharmacology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

• YOSHIMURA Yoshitaka

  Department of Molecular Cell Pharmacology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

• SUZUKI Kuniaki

  Department of Molecular Cell Pharmacology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

• TEI Kanchu

  Department of Oral and Maxillofacial Surgery, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

• TOTSUKA Yasunori

  Department of Oral and Maxillofacial Surgery, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

Abstract

We investigated the possible roles of estrogen on plasma membrane Ca²⁺-ATPase (PMCA) in human fibroblast-like synovial cells (HFLS) and mouse macrophage-like cells (RAW 264.7). Western blots revealed the expression of PMCA 2 and 4 in both cells. In vitro treatments with 17β-estradiol for 24 hours resulted in a concentration dependent decrease in PMCA expression. Moreover, Ca²⁺-ATPase specific activity was similarly decreased with estrogen treatments. However, treatments for 1 hour in the presence or absence of cycloheximide demonstrated non-significant effects. These results suggest that estrogen has a modulatory role on Ca²⁺ homeostasis through decreasing PMCA expression and abating their activity.

Journal

• Endocrine Journal

  Endocrine Journal 57 (1), 93-97, 2010

  The Japan Endocrine Society

Keywords

• PMCA
• Estrogen
• RAW 264.7 cells
• HFLS cells
• Ca<SUP>2+</SUP>-ATPase activity

Details

• CRID: 1390282681274601856
• NII Article ID: 10029583516
• NII Article ID: 130004443571
• NII Book ID: AA10901436
• DOI: 10.1507/endocrj.k09e-247
• ISSN: 13484540
• ISSN: 09188959
• Web Site: http://www.jstage.jst.go.jp/article/endocrj/57/1/57_K09E-247/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010142115
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed